Table 2 Primers were used in cloning and characterizing the 5-HT2B gene.

From: Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)

Primer name

Primer sequence (5′–3′)

5-HT2B 3′Outer

TTCATCGCCAACGTCCTCATCTCG

5-HT2B 3′Inner

TTCAACAAGACCTTCAGACAAACG

5-HT2B 5′Outer

GATGACCAGCGGAATGTAGAAGCA

5-HT2B 5′Inner

GGCGTTCTGGGGAGTGGTCCAAAGA

5-HT2B-F

ATGCCCACCCTAGGGGACCTCAC

5-HT2B-R

TCAGAGGTGGTACTTCCTTGTGGTCTTGATGTC

Nest universal primer

CTAATACGACTCACTATAGGGC

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