Figure 1

Notch activation in Dclk1-positive gastric tuft cells accelerates BE progression. (A) Experimental scheme illustrating timepoints of tamoxifen induction and tissue collection for analysis. (B) Representative H&E pictures of the SCJ of pL2.Dclk1 and pL2.Dclk1.N2IC mice, respectively; scale bars = 200 µm. (C) Histopathologic scoring in pL2.Dclk1.N2IC compared to pL2.Dclk1 controls for metaplasia (6 + 3 n = 23 pL2.Dclk1.N2IC, n = 7 pL2.Dclk1 controls; ordinary two-way ANOVA, p = 0.029) and dysplasia (6 + 3 n = 20 pL2.Dclk1.N2IC, n = 7 pL2.Dclk1 controls; ordinary two-way ANOVA, p = 0.033). (D) Representative macroscopic pictures of the opened stomach of pL2.Dclk1 and pL2.Dclk1.N2IC mice, respectively; scale bars = 0.5 cm. (E) Macroscopic scoring in pL2.Dclk1.N2IC compared to pL2.Dclk1 controls (6 + 3 n = 14 pL2.Dclk1, n = 42 pL2.Dclk1.N2IC mice, ordinary two-way ANOVA, p = 0.0001; 6 + 6 n = 8 pL2.Dclk1, n = 22 pL2.Dclk1.N2IC mice, ordinary two-way ANOVA, p ≤ 0.0001). (F) Histopathologic scoring in pL2.Dclk1.N2IC mice compared to pL2.Dclk1 controls for the number of crypt fissions (6 + 3 n = 7 pL2.Dclk1, n = 19 pL2.Dclk1.N2IC mice, multiple t test, adj. p = 0.019; 6 + 6 n = 6 pL2.Dclk1, n = 9 pL2.Dclk1.N2IC mice, multiple t test, adj. p = 0.035); error bars indicate SEM.