Figure 1

Microwell-mesh platform assembly, cell seeding, and implantation in mice. (a,b) The Microwell-mesh consisted of an array of microwells fabricated from PDMS with a nylon mesh bonded over the microwell openings. Fabrication of the Microwell-mesh is described in detail here¹⁸, and an animation demonstrating its capacity to retain cell spheroids is provided here^24,25. (c,d) A single cell suspension was pelleted into the microwells by centrifugation, forming multiple microtissues, or microtumours at the bottom of the microwells. (e) The entire microwell-mesh platform, containing microtissues was transplanted subcutaneously onto the back of immunocompromised NSG mice. Images c-d were drawn in AUTOCAD, and image e was adapted from a free pixabay vector drawing²⁸.