Figure 4
Mouse cell-depleted PDX PCA cells in 3D microtumour culture. (a) LuCaP35-derived cells formed small aggregates, sometimes requiring more than a single day to amalgamate into a single microtumour per well. (b) LuCaP141-derived cells were the least consistent in forming a single discrete microtumour per microwell. Frequently small clumps or loose cells would surround each microtumour. (c) BM18-derived cells formed the largest microtumours. While none of microtumours consistently increased in size during in vitro culture, cells did remain viable (dye Live(green)/Dead(red)) over several days. Red arrow points to a region of a microtumour that has become tightly aggregated and compacted, while the blue arrows point to cell debris that has not been incorporated into the spheroid. Scale bars = 200 µm.
