Figure 1

Hematoxylin & eosin (HE) and basement membrane proteins staining of serous and fibrous surface of native BP and AR-BP. (A) HE staining demonstrating that AR scaffolds are completely acellular, while retaining native BP collagen organization. (B) Collagen IV immunofluoresence staining demonstrating that AR-BP scaffolds retain native Col IV staining on the serous surface and vascular lumen. Fibrous surface of both native and AR-BP scaffolds does not contain Col IV. (C) Laminin immunofluoresence staining demonstrating retention of native tissue laminin content and organization, on the serous surface and vascular lumen of AR-BP scaffolds.