Figure 3

Z-direction cell migration into AR-BP scaffolds following serous or fibrous seeding. (A) HE images demonstrating cells remain predominantly as a monolayer on the surface following serous seeding. Conversely, cell penetration into the scaffold is higher following fibrous side seeding. (B) Percentage of cells penetrated into scaffolds quantified from H&E images. Significantly less cells penetrate into the scaffold following serous seeding versus fibrous seeding, p < 0.001. (C) Distribution of depth of penetration into the scaffold for those cells which do migrate in the Z-direction, demonstrating that overall cell penetration depth from serous side is significantly less than from fibrous side, p < 0.01. Statistical analysis was performed using one-way ANOVA, n = 6 scaffolds per group.