Figure 4
Effect of AR-BP sideness on cell proliferation. (A) FUCCI staining and quantification (B) at 6 h culture, showing no seeding side does not significantly effect number of replicating cells at early time points. Statistical analysis was performed using one-way ANOVA (n = 6 scaffolds per group). (C) Alarma blue intensity of cells from 0 to 6 days. At later time points (4 days and 7 days), cell number is greater following serous side seeding than fibrous side seeding. (D) Dead cell staining showing the dead cell percentage in both culture has no significant difference at all time points. Statistical analysis for alarma blue intensity and dead cell percentage was performed using two-way repeated measures ANOVA and Tukey’s multiple comparisons post hoc test. Data points that are not connected by the same lower case letter are significantly different. (n = 6 scaffolds per group).
