Figure 6
Effect of integrin blocking antibodies on cell migration and gene expression. Anti-α3β1 represents culture with integrin α3β1 antibody added; anti-α11 represents culture with integrin α11 antibody added; control represents culture without any antibody addition. (A) Addition of anti-integrin α3β1 (20 µg/mL) on serous side cell culture, significantly reduces cell migration. Additionally, expression of ITGA3 and ITGA11 are reduced following anti-integrin α3β1 antibody blockade. (B) Addition of anti-integrin α11 (20 µg/mL) on serous-side cell culture, significantly reduces cell migration only at later time points (i.e., 24 h). Expression of ITGA3, is reduced following anti-integrin α11 antibody blockade. (C) Addition of anti-integrin α3β1 (20 µg/mL) on fibrous side cell culture results in no significant difference in cell migration. Expression of ITGA3 is reduced following anti-integrin α3β1 antibody blockade. (D) Addition of anti-integrin α11 (20 µg/mL) on fibrous-side cell culture results in no significant difference in cell migration. However, expression of ITGA3 is reduced following anti-integrin α11 antibody blockade. Statistical analysis was performed using one-way ANOVA with repeated measures for time series data (n = 6 scaffolds per group; *p < 0.05; **p < 0.01). Data points that are not connected by the same lower case letter are significantly different.
