Figure 4 | Scientific Reports

Figure 4

From: Comparison of E. coli based self-inducible expression systems containing different human heat shock proteins

Figure 4

The SDS-PAGE analysis of EGFP expression in inducible and autoinducible systems. (a) Hsp27 SILEX system [lane 1: 2 h after inoculation, Lane 2: 16 h after inoculation, and lane 3: protein marker], (b) Hsp40 SILEX system [lane 1: 2 h after inoculation, Lane 2: 16 h after inoculation, and lane 3: protein marker (kDa)], (c) hsp70 SILEX system [lane 1: 2 h after inoculation, Lane 2: 16 h after inoculation, and lane 3: protein marker], and (d) Inducible system [lane 1: after induction, lane 2: before induction, and lane 3: protein marker]. The green arrow indicates the expression of EGFP and the red arrow indicates the leaky expression of the Hsp27, Hsp40, and Hsp70 on the gel a, b, and c, respectively. The protein marker also shows proteins with the molecular weights of 180, 135, 100, 75, 63, 48, 35, 25, 17, 11 kDa.

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