Figure 5 | Scientific Reports

Figure 5

From: Comparison of E. coli based self-inducible expression systems containing different human heat shock proteins

Figure 5

The SDS-PAGE analysis of expression of romiplostim and interleukin-2 in the SILEX systems. (a) Romiplostim: Hsp27 SILEX system [lane 2: 2 h after inoculation, Lane 3: 16 h after inoculation], Hsp40 SILEX system [lane 4: 2 h after inoculation, Lane 5: 16 h after inoculation], Hsp70 SILEX system [lane 6: 2 h after inoculation, Lane 7: 16 h after inoculation], and lane 1: protein marker. (b) Interleukin-2: Hsp27 SILEX system [lane 2: 2 h after inoculation, Lane 3: 16 h after inoculation], Hsp40 SILEX system [lane 4: 2 h after inoculation, Lane 5: 16 h after inoculation], Hsp70 SILEX system [lane 6: 2 h after inoculation, Lane 7: 16 h after inoculation], and lane 1: protein marker. The red arrow indicates the expression of romiplostim and interleukin-2 and the green arrow indicates the leaky expression of Hsp27, Hsp40, and Hsp70 on the gel a, b, and c, respectively. The protein marker also shows proteins with the molecular weights of 180, 135, 100, 75, 63, 48, 35, 25, 17, 11 kDa.

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