Figure 1
Experimental set up of peripheral focused ultrasound stimulation (pFUS). (A) Schematic showing pFUS targeting of the liver created using BioRender (https://biorender.com/icon/species/rodents/mouse-supine-with-organs/). Anesthetized mice are laid on the supine position, while the conical focused ultrasound transducer is aimed at the porta hepatis. Region of stimulation is represented by the yellow circle. (B) Experimental timeline for daily stimulation. Mice are given 8 weeks to establish either the Western diet or the control diet, then are subjected to hepatic pFUS from weeks 9–16, with weekly blood draws. At the end of week 16, mice are euthanized and tissues are harvested. C. Experimental timeline for alternate-day stimulation. The alternate-day stimulation paradigm is identical to the daily stimulation, except mice receive stimulation every other day for weeks 9–16.
