Figure 5

Signal intensity (n = 3) of LAM from whole sheep’s blood separated on the microfluidic device vs. benchtop methods. Higher intensity indicates higher concentration of biomarker retained in the serum sample. There was no significant difference between signals, indicating that our cross-flow filtration chip is suitable for the separation of serum from blood and subsequent detection of amphiphilic biomarkers. The fact that serum processed on the microfluidic disc yielded a similar signal intensity indicates that the microfluidic device preserves amphiphilic biomarkers present in serum as effectively as benchtop processing methods.