Figure 2 | Scientific Reports

Figure 2

From: Adventitial delivery of nanoparticles encapsulated with 1α, 25-dihydroxyvitamin D3 attenuates restenosis in a murine angioplasty model

Figure 2

Decreased IER3 and CCL2 gene and protein expression in PTA plus 1,25 NP treated vessels. (A) Representative IER3 and CCL2 staining at day 21 in the 1,25 NP treated outflow graft veins and vehicle control veins, respectively. (B, C) At day 3, there was a significant decrease in the average gene expression of Ier3 and Ccl2 of 1,25 NP treated GVs compared to vehicle controls, respectively. (D, E) Semiquantitative analysis demonstrated significant decreases in the average IER3 and CCL2 indexes in 1,25 NP treated vessels compared to controls. Each data point in the scatter plot bar graph represents the mean ± SEM of 3–6 animals. Unpaired t-test was performed. Significant differences between groups are indicated *p < 0.05, **p < 0.01, ***p < 0.001. Cells staining positive for IER3 and CCL2 are brown staining. L lumen, ADV adventitial; solid arrows, positive cells. Scale bar is 50 μm.

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