Table 5 Polymorphism number, annealing temperature and PIC of studied Thymus species.
From: Polyphenolic and molecular variation in Thymus species using HPLC and SRAP analyses
No | Primer | Sequence (5′ → 3′) | Annealing tempa | primer combinations | No. total bands | No.PBb | PPBc (%) | PIC |
|---|---|---|---|---|---|---|---|---|
1 | Me1 | 5′-TGAGTCCAAACCGGATA-3′ | 50 | Me1 + em4 | 15 | 14 | 93.33 | 0.38 |
2 | Me2 | 5′-TGAGTCCAAACCGGAGC-3′ | 50 | Me2 + em1 | 18 | 16 | 88.88 | 0.32 |
3 | Me3 | 5′-TGAGTCCAAACCGGAAT-3′ | 50 | Me2 + em3 | 13 | 13 | 100 | 0.47 |
4 | Me4 | 5′-TGAGTCCAAACCGGACC-3′ | 50 | Me3 + em1 | 13 | 12 | 92.30 | 0.33 |
5 | Me5 | 5′-TGAGTCCAAACCGGAAG-3′ | 50 | Me4 + em3 | 10 | 10 | 100 | 0.23 |
6 | Em1 | 5′-GACTGCGTACGAATTAAT-3′ | 50 | Me5 + em3 | 14 | 13 | 92.85 | 0.28 |
7 | Em3 | 5′-GACTGCGTACGAATTGAC-3′ | 50 | Me5 + em4 | 19 | 17 | 89.47 | 0.38 |
8 | Em4 | 5′-GACTGCGTACGAATTTGA-3′ | 50 | |||||
Total | 102 | 95 | – | – | ||||
Average | 14.57 | 13.57 | 93.83 | 0.34 |
