Figure 5 | Scientific Reports

Figure 5

From: Mice lacking PLAP-1/asporin counteracts high fat diet-induced metabolic disorder and alveolar bone loss by controlling adipose tissue expansion

Figure 5

Adipocyte differentiation of 3T3-L1 cells in the presence of recombinant PLAP-1. (A) PLAP-1 expression in culture supernatants from 3T3-L1 cells infected with adenoviruses that carried LacZ or mouse Plap-1 was identified by Western blot analysis. Total protein level was shown by Ponceau S. Control CM: Control conditioned medium infected with adenoviruses that carried LacZ, PLAP-1 CM: PLAP-1 conditioned medium infected with adenoviruses that carried Plap-1. The original blot images are included in Fig. S4. (B) Oil Red O staining of 3T3-L1 cells in the presence of recombinant PLAP-1 was conducted after adipocyte differentiation. (C) Quantitative lipid assay of 3T3-L1 cells in the presence of recombinant PLAP-1 was performed after Oil Red O staining (n = 4). Values represent the mean ± SD. (D) Real-time PCR analysis was performed to examine adipogenic related gene expression of 3T3-L1 cells in the presence of recombinant PLAP-1 during adipocyte differentiation (n = 3). Results show the mean ± SD of triplicate assays. *:p < 0.05, **: p < 0.01.

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