Table 1 Ranking of the candidate reference genes according to their stability value calculated by NormFinder.

From: Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues

Rank

Somatic embryogenesis group

Germination group

Gene name

Stability value

Gene name

Stability value

1

EF1g

0.0024

EF1g

0.0013

2

HIS1

0.0046

ACT

0.0016

3

GAPDH

0.0055

Rpl2

0.0030

4

TUA

0.0063

HIS1

0.0035

5

Rpl2

0.0088

EF1a

0.0062

6

ACT

0.0092

GAPDH

0.0066

7

UBQ

0.0102

UBQ

0.0075

8

EF1a

0.0119

TUB

0.0097

9

TUB

0.0126

TUA

0.0114

10

18S rRNA

0.0210

18S rRNA

0.0190

  1. The input data for NormFinder are supposed to be on a linear scale. The raw Ct values were transformed to linear scale expression quantities using the standard curve or delta-Ct method.