Table 5 Descriptions of Liriodendron hybrids’ candidate reference genes and associated qPCR analyses.
Symbol | Gene description/function | GenBank no | Primer sequences | Tm (°C) | Amplicon (bp) | Slop | R2 | E (%) |
|---|---|---|---|---|---|---|---|---|
Rpl2 | Ribosomal protein L2/Protein synthesis | AF123796 | F:5′-ACACCAATCCATCCCGAACT-3′ | 79.73 | 93 | − 3.494 | 0.999 | 93.3 |
R:5′-TCCTGGCGTCGAGCTATTT-3′ | ||||||||
18S rRNA | 18S ribosomal RNA/Constituent of the small ribosomal subunit | FR870008 | F:5′-ATTTCTGCCCTATCAACTTTCG-3′ | 82.42 | 183 | − 3.466 | 0.998 | 94.5 |
R:5′-TTGTTATTTATTGTCACTACCTCC-3′ | ||||||||
ACT | Actin/Cytoskeletal structural protein | GQ246181 | F:5′-CTCGGCTGTGGTTGTGAAG-3′ | 82.32 | 160 | − 3.469 | 0.996 | 94.2 |
R:5′-TGGTGTGATGGTTGGTATGGG-3′ | ||||||||
EF1a | Elongation factor 1-alpha /Protein synthesis | FR870012 | F:5′-ATCATGAATCACCCAGGACA-3′ | 80.27 | 147 | − 3.017 | 0.954 | 104.5 |
R:5′-TTCAAGAACTTGGGCTCCTT-3′ | ||||||||
EF1g | Elongation factor 1-gamma/Protein synthesis | FD496784 | F:5′-ATCCGGGATTCACTTGGATA-3′ | 75.85 | 114 | − 3.349 | 0.998 | 98.9 |
R:5′-CGTCACCCATAACTTTGCAC-3′ | ||||||||
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase/metabolic function | FD495585 | F:5′-ACAACTAACTGCCTTGCTCCTT-3′ | 83.5 | 274 | − 3.263 | 0.997 | 102.5 |
R:5′-AGTCAGATCCACCACCGAAA-3′ | ||||||||
HIS1 | Histone H1 linker protein/Compacting DNA strands and Chromatin regulation | CO995299 | F:5′-AAGCCGAAGAAGGCGAAAGC-3′ | 81.93 | 100 | − 3.137 | 0.986 | 108.3 |
R:5′-TCTCGAAGTAGGGCGGATGG-3′ | ||||||||
TUA | a-Tubulin/Cytoskeletal structural protein | FD499140 | F:5′-TGACTGGAGCATAAGATGAAAGC-3′ | 80.46 | 153 | − 3.300 | 0.983 | 100.9 |
R:5′-CCAATCTCAACCGCCTCG-3′ | ||||||||
TUB | β-Tubulin/Cytoskeletal structural protein | FD493593 | F:5′-TCTCCGCCTCCGTAAACTC-3′ | 80.64 | 140 | − 3.330 | 0.991 | 99.7 |
R:5′-ATTCATTGGGAACTCAACATCG-3′ | ||||||||
UBQ | Ubiquitin/Protein degradation | CK755254 | F:5′-GGCATTCCACCAGACCAGC-3′ | 82.49 | 131 | − 3.118 | 0.998 | 109.3 |
R:5′-TGCATCCCGCCCCTCAAT-3′ |
