Figure 1

Morphology of MaSm and MaRg colony-biofilms. (A) MaSm and (B) MaRg were grown on nitrocellulose membranes for 4 days, transferring the membranes onto fresh media after 48 h. Colony-biofilms were then imaged to visualize the macroscopic morphology. Scale bar indicates 5 mm, and 0.5 mm for the zoomed insets. (C) To assess the biomass and if each variant was stable during biofilm formation, 4 day M. abscessus biofilms were enumerated for CFUs. Evidence of phenotypic changes of the colony morphologies for either was not observed at 37ÂşC over 4 days. Statistical analysis was performed using a Studentâs t-test; ns indicated not significant. Nâ=â3.