Figure 2 | Scientific Reports

Figure 2

From: A suitable RNA preparation methodology for whole transcriptome shotgun sequencing harvested from Plasmodium vivax-infected patients

Figure 2The alternative text for this image may have been generated using AI.

qRT-PCR of P. vivax total RNA samples. qRT-PCR amplification of housekeeping genes seryl tRNA synthetase (a,d), DNA repair helicase (b,e) and gamma-glutamylcysteine synthetase (c,f), from the RNA samples of one Pv-iRT field-isolate (103 in light gray to 106 in dark gray), preserved in RNAlater or TRI Reagent and extracted by RNeasy Micro kit (a–c) or Direct-zol RNA MiniPrep (d–f), respectively. Bars and error bars represent the mean Cq and standard deviations (SD), respectively. Black square marks represent average Cq of RT- reactions, when amplification was observed before the last (45th) cycle stage. Blue diamond marks represent mean Cq amplification of human TLR9 gene for host contamination detection. Black * indicate when the mean Cq for each sample is higher than the limit of detection (3 times the Cq SDs from the correspondent RT- mean Cq) and blue § indicate when the mean Cq is higher than the mean Cq for host contamination detection (3 times the Cq SDs from the correspondent TLR9 mean Cq).

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