Table 1 Kinetic analysis of mAbs using bio-layer interferometry (BLI).

From: In vitro and in vivo inhibition of malaria parasite infection by monoclonal antibodies against Plasmodium falciparum circumsporozoite protein (CSP)

mAb

Fab binding NANPx6 target antigen

Fab binding FL-CSP antigen

Bivalent mAb binding FL-CSP target antigen

Ratio of Fab KD NANPx6/CSP

Ratio of KD Fab/mAb

KD (nM)

ka (M−1 s−1)

kd (s−1)

KD (nM)

ka (M−1 s−1)

kd (s−1)

KD (nM)

ka (M−1 s−1)

kd (s−1)

311

7

2.7E+04

2.0E−04

0.2

1.0E+05

2.1E−05

0.07

4.8E+05

3.07E−05

35.8

3

317

0.003

5.9E+04

1.7E−07

0.002

1.2E+05

2.6E−07

0.03

1.1E+06

2.97E−05

1.3

0.08

CIS43

41

4.0E+04

1.7E−03

2

2.8E+04

4.8E−05

0.46

1.5E+06

6.92E−04

24.4

4

MGG4

51

9.0E+04

4.5E−03

47

5.1E+04

2.4E−03

0.17

4.7E+05

8.16E−05

1.1

275

663

138

4.0E+05

5.6E−02

139

1.3E+05

1.8E−02

1

9.7E+05

1.12E−03

1.0

120

580

2650

1.5E+05

3.9E−01

524

1.9E+05

9.7E−02

6

2.1E+06

1.29E−02

5.1

83

  1. Equilibrium dissociation constant (KD), association rate constant (ka), and dissociation rate constant (kd) of the antigen-binding fragment (Fab) and intact monoclonal antibodies (mAbs). Ratios represent KD values of Fab binding to NANPx6 / FL-CSP antigen; and ratio of KD values of Fab / intact mAbs binding to FL-CSP.
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