Table 2 Correlation analysis.

From: In vitro and in vivo inhibition of malaria parasite infection by monoclonal antibodies against Plasmodium falciparum circumsporozoite protein (CSP)

Protection study

Expt 1 (24 h)

Expt 1 (48 h)

Expt 2 (24 h)

ELISA (FL-CSP)

0.27 (0.30)

0.75 (0.00)

0.26 (0.28)

ELISA (NANPx6)

− 0.54 (0.14)

− 0.73 (0.01)

− 0.14 (0.58)

ELISA (SPZ)

− 0.47 (0.21)

− 0.60 (0.08)

0.46 (0.02)

ILSDA (0.5 µg/ml)

0.54 (0.00)

0.41 (0.11)

0.71 (0.01)

Avidity Index FL-CSP

0.72 (0.01)

0.68 (0.01)

0.59 (0.03)

Avidity Index NANPx6

0.68 (0.02)

0.78 (0.00)

0.67 (0.02)

Avidity (FL-CSP)

0.77 (0.00)

0.53 (0.12)

0.84 (0.00)

Avidity (NANPx6)

0.67 (0.03)

0.77 (0.00)

0.65 (0.02)

KD Bivalent (FL-CSP)

− 0.55 (0.18)

− 0.80 (0.01)

− 0.26 (0.46)

KD Fab (FL-CSP)

− 0.56 (0.25)

− 0.82 (0.05)

− 0.14 (0.86)

KD Fab (NANP6)

− 0.56 (0.25)

− 0.73 (0.10)

− 0.34 (0.66)

BSA (NPNA)n

0.84 (0.04)

0.81 (0.05)

0.36 (0.64)

BSA junctional

0.65 (0.17)

0.59 (0.22)

0.20 (0.80)

  1. Pearson’s correlation coefficient r and the level of significance (bracket) between in vivo challenge (Experiment 1 and 2) and in vitro assays. Assays included FL-CSP ELISA; NANPx6 ELISA; Sporozoite ELISA; ILSDA at 0.5 µg/ml; Avidity Index (AI) against FL-CSP; AI against NANPx6; Avidity against FL-CSP; Avidity against NANPx6; KD Bivalent against FL-CSP; KD Fab against FL-CSP; KD Fab against NANP6; Buried Surface Area between mAb and (NPNA)n epitope and BSA against junctional epitope. Pearson's correlation coefficient |r|> 0.7 suggested a strong linear association; |r|= 0.4–0.7 suggested moderate linear association; and no linear association was inferred for |r|< 0.4. Bold values are |r|> 0.7 (p < 0.05).
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