Figure 1

PCA scatter plot of the metabolite profile. The HTH3d group is separated from the NC3d (A), HTH10d and NC10d (B) components. OPLS-DA analyzes the metabolite profile. The OPLS-DA score graph (C) and OPLS-DA substitution test graph (D) show a good distinction between the HTH3d group and the NC3d group, R2X = 0.311, R2Y = 0.978, Q2 = 0.907, p < 0.05. The OPLS-DA score graph (E) and OPLS-DA substitution test graph (F) show a good distinction between the HTH10d and NC10d groups, R2X = 0.439, R2Y = 0.965, Q2 = 0.638, p < 0.05.