Figure 3
From: GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
Relative expression levels of GFAT (A) and PFK (B) after dsGFAT and dsPFK injection, and phenotype changes (C) after dsRNA injection in Nilaparvata lugens. GFAT, glutamine:fructose-6-phosphate aminotransferase; PFK, phosphofructokinase. The N. lugens used for the microinjection of RNAi were at the 1st day of 5th instar nymph stage. dsGFAT or dsPFK injection were used as the test groups whereas dsGFP injection was used as the control group. Gene expression levels were measured by quantitative real-time PCR with 18S RNA as the internal control. Values are means ± SE from three independent measurements. Different letters indicate significant differences according to Duncan’s test (p < 0.05).
