Figure 5
From: GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
Relative expression levels of chitin biosynthesis-related genes at 48 h and 72 h after dsRNA injection. GFAT, glutamine:fructose-6-phosphate aminotransferase; PFK, phosphofructokinase; GAPNA, glucosamine-6-phosphate N-acetyltransferase; PGM, phosphoacetylglucosamine mutase; UAP, UDP-N-acetylglucosamine pyrophosphorylase. The expression of GFAT (A), GNPNA (B), PGM1 (C), PGM2 (D), and UAP (E) was determined by quantitative real-time PCR with 18S RNA as the internal control. The N. lugens used for RNAi microinjection were at the 1st day of the 5th instar nymph stage and dsGFAT or dsPFK injection were used as test groups whereas the dsGFP injection was used as the control group. Values are the average of three sets of data and standard errors were calculated. *, significant differences (P < 0.05); **, extremely significant differences (p < 0.01).
