Table 1 Gene-specific primers used for quantitative real-time polymerase chain reaction (qRT-PCR).
From: GFAT and PFK genes show contrasting regulation of chitin metabolism in Nilaparvata lugens
Primer nnnnanamename | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
QNl18S | CGCTACTACCGATTGAA | GGAAACCTTGTTACGACTT |
QNlGFAT | CTGGACTTTGACAGCGTTAC | GTGGTCGTTGTCGGAGC |
QNlPFK | TGACGTGACAGGGTGGGT | ATGGCTTGGATTTGGAACT |
QNlGNPNA | TGAGCTGCTGAAGACACT | CCTGAATAACGGTGATGTA |
QNlPGM1 | AACGAGACGGTGGGAGAC | TCCTGGTAAGTGTTGAGCC |
QNlPGM2 | AGAGGAAGGTTGGGAGTG | CATAATTCGCGGAGATAAG |
QNlUAP | ACGACAGATTAAAGCCGATAC | TACCTTGTCCACCAGCCA |
QNlCht1 | AGGTGGTTAGGGACGAGGAG | TGCGCTTGACATAGTTGGACT |
QNlCht2 | GCAGATTTCTGGACAGGGAA | TGACGCACAAGCGGGAAG |
QNlCht3 | CTACACCTCTGGCTAAACTCGG | AACTTGTCCTTGCGGCTGAT |
QNlCht4 | TTGAGGAGGTTCACGGGTCT | CCTTACTGGAAACGAGGTTGG |
QNlCht5 | AAAGCGTTCGTGATGAAATAGC | GATCCTTTGCCTCAATCCAAT |
QNlCht6 | GCTGGTAAGGAGATGCTATTCG | GTGGTTCTAAGGCTGGCTGTC |
QNlCht7 | CTACTCTGCCATCCCATTCCT | GTCTGGGTTTCTTCACTTCCTG |
QNlCht8 | GAACAAAGTGCAAACTCAGTCC | CACCTTCTGTGGCTTCTGG |
QNlCht9 | GTGCGGTATTGGTTGAAGAGG | GGTATAACGTGATTCCGAGCC |
QNlCht10 | CAAGCCAATACCCAACAAAC | ACAGCAAATCCATAGAGCACA |
QNlIDGF | AAAAGAACGAGGAGGAGGG | TTGCTTGAGGATGGGGTAC |
QNlENGase | TGTGGCAAGACTTCGTTA | ATGGGAGGGTTGGGATAG |
QNlCHS1 | CCGCAAACGATTCCTACAGA | AGGTCCTTGACGCTCATTCC |
QNlCHS1a | TGTTCTTGCTACAACTCAATAAA | ACACCAATCCGATAGGCTC |
QNlCHS1b | GCTGTCTTTGCTTTCTTCAT | ACACCAATCCGATAGGCTC |
