Figure 1
From: Pharmacological targeting of host chaperones protects from pertussis toxin in vitro and in vivo
(a) Effect of BFA, Rad, FK506 and CsA on the intoxication of CHO-K1 cells with PT. CHO-K1 cells were pre-incubated with 10 µM BFA, Rad, FK506 or CsA or left untreated for control. After 30 min 10 ng/ml PT were added. 1.5 h later the culture medium was removed, and cells were further incubated at 37 °C and 5% CO2 in fresh medium that did not contain PT or any inhibitor. Pictures were taken after 18 h. A quantitative analysis of total cell number of CHO-K1 cells is shown, values are normalized on control cells (n = 3, mean ± SD). (b) Pharmacological inhibition of Hsp70 activity protects cells from PT-intoxication. CHO-K1 cells were treated with VER (30 μM) or HA9 (20 µM) for 30 min and then intoxicated with PT (10 ng/ml) for 18 h. Pictures were taken, and cell numbers were determined as described in A. Significance was tested by one-way ANOVA with Dunnett’s multiple comparisons test and refers to samples treated with PT only (*p < 0.05, **p < 0.01, ***p < 0.001). Scale bar = 50 µm.
