Figure 3

Evaluation on coculture effects in PXB-cells. (A) Morphology of PXB cells in monoculture and coculture. The white arrows point to bile canaliculi structures. (B) Enhancement in liver function in PXB-cells by coculture with iPS-intestinal cells. The CYP activities using each specific substrate in monoculture and coculture of PXB-cells were measured for 48 h (from day 6 to day 8 in Fig. 1D). Each bar represents the mean ± SD (n = 8) from two independent experiments normalized to the value obtained from the monoculture. (C) Albumin concentration in culture medium measured on day 8 (Fig. 1D). Each bar represents the mean ± SD (n = 11) from two independent experiments. (D) Relative gene expression of PXB-cells. Each data point represents the mean ± SD (n = 7) from two independent experiments. Each value is normalized to the value obtained from the monoculture of each tested gene. In all panels, *p < 0.05, **p < 0.01, and ***p < 0.001. (E) Phase II drug metabolising enzyme related gene expression in PXB-cells. Each data point represents the mean ± SD (n = 10–11) from two independent experiments. Each value is normalized to the value obtained from the monoculture of each tested gene. In all panels, *p < 0.05, **p < 0.01, and ***p < 0.001.