Figure 3

Antibody footprints on the capsid surface of EV-A71 and CV-A16. The capsid surfaces of EV-A71 and CV-A16 are shown as stereographic projections. An icosahedral subunit is outlined by the triangular boundary and the locations of five, three, and twofold vertex are marked as black pentagons, triangles and ovals, respectively. (A,B) The residues from VP1 are shown in light pink, VP2 in light blue and VP3 in light orange, respectively; the residues in conformational epitopes site 1, site 2 and site 3 are colored in red, green and blue, respectively. (A) The binding sites of seven neutralizing antibodies on EV-A71 are indicated by solid lines in different colors (Mab 28-7: yellow, D5: purple, 10D3: aquamarine, D6: brown, A9: gray, E18: deep pink, E19: orange). (B) The binding sites of three antibodies 18A7, 14B10 and NA9D7 on CV-A16 are marked by blue, red and white solid lines, respectively. (C,D) The surfaces of EV-A71(C) and CV-A16 (D) are colored radially from blue through green to red from the lowest to the highest. The binding sites of all antibodies of these two serotypes are also outlined, respecting the color rules in (A,B).