Figure 4
From: STAT3 governs the HIF-1α response in IL-15 primed human NK cells
STAT3 inhibition interferes with CCL3/CCL4 secretion and IFNγ production in NK cells. NK cells were incubated under normoxia or hypoxia for 16 h and treated with IL-15 alone (A − C) or in combination with IL-12/IL-18 (D) for additional 6 h in the presence or absence of STAT3 inhibitors (S3I-201, stattic) as indicated. (A − C) Bead array analysis of secreted chemokines. Bars represent mean chemokine concentrations ± SD (n = 3). The level of statistical significance was not reached when applying the Wilcoxon signed-rank test. ND: not detectable. (D) Pooled flow cytometric data for the proportions of IFNγ median fluorescence intensities (MFI), shown as mean values ± SD (n = 4). The level of statistical significance was not reached when applying the Wilcoxon signed-rank test.
