Figure 6
From: Gut microbiota markers associated with obesity and overweight in Italian adults
Statistically significant differences in predicted metabolic pathways between OB and NW. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) algorithm was performed on Galaxy software v.1.0. (https://galaxy.morganlangille.com/) to infer metagenome composition in the samples by analyzing OTUs generated by QIIME pipeline. Bacterial metabolic pathways were predicted and classified by Kyoto Encyclopedia of Genes and Genomes (KEGG). Statistically differences were analyzed for all metabolism pathways using the Statistical Analysis of Metagenomic Profiles (STAMP) software. Pathways more abundant in OB are on the positive side (light-blue circle with 95% CI); pathways less abundant in OB are on the negative side (pink circle with 95% CI). The statistical significance was tested using Welch’s test, with a Storey False Discovery Rate (FDR) correction. q equal to or less than 0.05 was considered statistically significant. Mean proportions are shown in stacks (OB = light- blue; NW = pink). The difference in mean proportions indicates the mean proportion OB minus the mean proportion NW.
