Figure 1
From: Collective migration during a gap closure in a two-dimensional haptotactic model
Epithelial gaps closure is slowed down on a FN gradient compared to on homogeneously coated substrates. (A) Design of the photopattern used to create a centered circular gradient of FN (gradient of gray) within a square FN pattern (in white) of 764 µm wide. (B) Epifluorescent image of a FN gradient labelled with rhodamine. (C) Plot profile of the fluorescence intensity (left axis) and the surface density (right axis) of the rhodamine-conjugated FN according to the blue arrow. Low fluorescence intensity values at both extremities correspond to the black frame of (B). (D) Time-lapse images in DIC mode of a gap closure in a confluent epithelium grown on a circular FN gradient. The edge of the closing gap was highlighted with a black line. Scale bar, 100 µm. Distribution of (E) the gap area and (F) the gap perimeter in confluent epithelia grown on a uniform coating (in black) or a gradient (in blue) of FN. (G) Temporal evolution of the mean normalized gap area during the closure on a uniform coating (in black, n = 7) or a gradient (in blue, n = 6) of FN. Full lines represent the mean and dashed lines the S.D.
