Figure 1 | Scientific Reports

Figure 1

From: Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile

Figure 1Figure 1

Growth and characterization of primary epithelial cells. (a) Contrast phase images were taken 3, 6 and 10 days after seeding the cells. (b,c) Cells seeded were fixed and stained with anti-human E-cadherin (b) or with anti-human cytokeratin 14 or 18 (c) antibodies and fluorochrome-conjugated secondary antibodies. DNA was labeled with Hoechst. (d) Epithelial cells and fibroblasts were isolated from one explant and stained (right) or not (left) with FITC-conjugated anti-human E-cadherin antibody, and analyzed by flow cytometry. All experiments were performed using primary epithelial cells isolated from at least 3 donors.

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