Figure 5

Inflammatory response of HeLa and primary epithelial cells upon Chlamydia infection. (a) HeLa and primary epithelial cells were infected with C. trachomatis serovar L2 (MOI = 1 for HeLa cells and MOI = 10 for primary epithelial cells, respectively). Thirty hours post infection, RNAs were extracted from cell lysates and the transcripts for the indicated genes were measured by real-time quantitative PCR and normalized to house-keeping gene β-actin following the 2^−ΔΔCt method. (b) HeLa and primary epithelial cells were infected with Chlamydia serovar D (bacterial stock diluted 1:500 for HeLa cells and 1:50 for primary epithelial cells). Forty-eight hours post infection, the samples were processed as in (a). The data are presented as Log2 fold changes of mRNA compared to uninfected HeLa cells. Each plot displays data obtained from at least 3 independent experiments and each individual point in the primary cell group represents the results of cells isolated from a different donor. The data were presented as mean ± SEM and the non-paired Student’s t-test was used for two group comparisons. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.