Figure 3 | Scientific Reports

Figure 3

From: Profiling of three H3F3A-mutated and denosumab-treated giant cell tumors of bone points to diverging pathways during progression and malignant transformation

Figure 3

Morphology of Case 3. (1) Biopsy. (2) Resection specimen. (3) Sarcoma. Typical GCTB with osteoclastic giant cells and intermingled mononuclear cells in the biopsy (1A) and the resection specimen (2A) as well as neoplastic mononuclear cells with positive H3F3A G34W staining in the nuclei (1B,2B) (bar = 100 µm). Staining for Ki-67 shows about 1% of positive cells (1C,2C). Sanger-Sequencing for H3F3A G34W shows the mutation specific thymine in tissue of the biopsy (1D) as well as the resection specimen (2D). The sarcoma showed spindle to pleomorphic tumor cells and extensive necrosis (3A) and a high Ki-67 index (3C), however it did not stain positive for H3F3A G34W (3B). Sanger-sequencing of DNA from microdissected tissue of the H3F3A G34W-negative tumor showed a very weak peak for a mutation-specific thymine (3D).

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