Figure 2
From: Metabolic engineering of probiotic Escherichia coli for cytolytic therapy of tumors
HlyE production and functional activity. (a)The SDS-PAGE analysis of HlyE. The production of HlyE in either EcNe/HlyE (top) or EcN/HlyE (bottom) was conducted with LB medium. The arrow indicates the location of HlyE in SDS-PAGE. Lane 1, without induction (−); lane 2, induction (+)/10 μM L-arabinose; lane 3, (+)/30 μM L-arabinose; lane 4, (+)/50 μM L-arabinose; lane 5, (+)/100 μM L-arabinose; lane 6, (+)/1 mM L-arabinose; lane 7, (+)/10 mM L-arabinose. (b) The effect of glucose on the HlyE production in EcNe/HlyE. EcNe/HlyE was grown on LB medium plus various amounts of glucose and induced for the HlyE production by adding 50 μM L-arabinose. Lane 1, (−)/nil glucose; lane 2, (+)/nil glucose; lane 3, (+)/glucose of 4 g/100 ml; lane 4, (+)/glucose of 10 g/100 ml. (c) The effect of glucose on the HlyE production in EcN/HlyE. EcN/HlyE was grown on LB medium plus various amounts of glucose and induced for the HlyE production by adding 1 mM L-arabinose. Lane 1, (−)/nil glucose; lane 2, (+)/nil glucose; lane 3, (+)/glucose of 4 g/100 ml; lane 4, (+)/glucose of 10 g/100 ml; lane 5, (+)/nil glucose; lane 6, (+)/glucose of 0.1 g/100 ml; lane 7, (+)/glucose of 0.2 g/100 ml.
