Figure 4
From: Metabolic engineering of probiotic Escherichia coli for cytolytic therapy of tumors
In vitro toxicity of EcNe/HlyE. (a) The specific attachment of EcNe to tumor cells. (b) Examination of cell viability by MTT assay. SW620 or HT29 cells were incubated with either EcNe or induced EcNe/HlyE at various MOI as indicated for 3 h. (c) Time-lapse images of cell nuclear morphology by DAPI staining. Images of SW620 and HT29 cells were taken over time as indicated on the top upon administration with either EcNe or induced EcNe/HlyE at MOI of 100. Bacteria were identified in red. (d) Time-lapse images of cell nuclear morphology by PI staining. Arrows indicate internal rearrangement of organelles and loss of membrane integrity. Cell images were processed with GEN5 3.0 software (Biotek).
