Figure 4
From: High intraluminal pressure promotes vascular inflammation via caveolin-1
Pressure-induced endothelial activation is caveolin-1 dependent. (A) Transformed H5V cells were transfected with either scrambled shRNA (H5V shScr) or Cav1 shRNA (H5V shCav) (Full blot Figure S3). (B) Ccl2 and (C) Icam1 gene expression in H5V shScr (white bars) and H5V shCav (black bars) cells that were untreated (Ctr), or pressurised at 0 or 120 mmHg for 1 h (n = 4–6). (D) Nuclear and (E) cytosolic NFĸB fluorescence (normalized to shScr Ctr) was also quantified in H5V shScr and H5V shCav cells that were unpressurised (Ctr) or pressurised to 120 mmHg (n = 5). All results are expressed as mean ± SEM. Data were analysed with a two-way ANOVA (B-E) with Bonferroni post hoc test where *p < 0.05, p < 0.01 and ***p < 0.001.
