Figure 5
From: Adipose most abundant 2 protein is a predictive marker for cisplatin sensitivity in cancers
The APM2 expression in the liver tissue and chemosensitivity to CDDP. The representative computed tomography images before and after the transcatheter arterial infusion of cisplatin (CDDP) to the hepatocellular carcinoma (HCC) resulted in the partial response (PR), stable disease (SD) and progressive disease (PD). White arrows indicate the tumors. (a, b) PR case, (c, d) SD case, (e, f) PD case. White arrows indicate the tumor. (g–r) Representative histological analyses. Hematoxylin and eosin staining of surrounding liver tissue (SL) and HCC in PR case (g, h), SD cases (k, l) and PD case (o, p). Immunohistochemical staining of APM2 of SL and HCC in PR case (i, j), SD cases (m, n) and PD case (q, r). The scale bar represents 100 µm. (s) A quantitative analysis of positively stained area in SL and HCC tissues with APM2. For quantitative measurements, 3 representative images from each patient in 3 groups (PR, n = 4, SD, n = 6, PD, n = 3) were captured at a final magnification of × 200. Quantification was performed measuring the integrated density in pixels using the ImageJ software (version 1.6.0_20, National Institutes of Health, USA). The APM2 stained/negative control ratio of the integrated density in pixels was calculated as the relative integrated density. The values represent means ± standard deviations. APM2, adipose most abundant 2. **p < 0.01, ***p < 0.001, One-way analysis of variance followed by Bonferroni’s multiple comparison test. (t–v) The ERCC6L expression in the liver tissue and chemosensitivity to CDDP. The representative immunohistochemical staining of ERCC6L in the hepatocellular carcinoma (HCC) showed partial response (PR), stable disease (SD) and progressive disease (PD). (t) PR case, (u) SD case, (v) PD case. The scale bar represents 100 µm. (w) A quantitative analysis of positively stained cells with ERCC6L in 3 groups (PR, n = 4, SD, n = 6, PD, n = 3). Quantification was performed measuring the integrated density in pixels using the ImageJ software (version 1.6.0_20, National Institutes of Health, USA). The ERCC6L stained/negative control ratio of the integrated density in pixels was calculated as the relative integrated density. The values represent means ± standard deviations. *p < 0.05, ***p < 0.001, One-way analysis of variance followed by Bonferroni’s multiple comparison test.
