Figure 3

Effect of prediabetes and early phase type 2 diabetes on PGF2α levels in plasma and on the expression of PGF2α receptor. (a) PGF2α levels in plasma of control animals (n = 6) and animals submitted to HF (n = 4) or HFHSu (n = 6) diets. (b) Average relative expression of PGF2α receptor (60KDa) and the loading protein β-Actin (43 kDa) or GAPDH (37 kDa) in aorta and pulmonary artery (PA) of control animals (n = 6) and animals submitted to HF (n = 6) and HFHSu (n = 6) diets. Representative western blots for each protein studied are depicted above the respective graphs. For PGF2α expression, the representative bands were cropped due to the sample order in the membrane. For complete membrane image see Supplementary data. Values are presented as scatter plots ± SD; One-way ANOVA with Dunnet’s comparison test. **p < 0.01 and ***p < 0.001 versus control animals.