Figure 1

Metabolomics assessment of follicular fluid constituents. (a) Schematic depicting the experimental design of the study with the collection of FF for metabolomics analysis and the corresponding oocyte for IVF. Resultant embryos were classified as uncleaved (N = 22), cleavage stage (N = 21), or blastocyst (N = 31). (b) The total number of significant metabolites (p ≤ 0.05) that were upregulated or downregulated in the FF between the embryo groups was determined through pairwise comparisons. Note that the majority of metabolites that were statistically different between the blastocyst and cleavage stage embryo groups were downregulated in blastocysts. (c) Pathway enrichment analysis of the metabolites in the FF shown as pairwise comparisons identified multiple fatty acid and metabolism pathways as well as the TCA cycle components that were different between the embryo groups. An enrichment score of greater than 1 indicates that the pathway is enriched in the first group listed compared to the second group and a value of less than 1 indicates that the pathway is enriched in the second group listed versus the first group. Enrichment score = (k/m)/((n-k)/(N-m)) where m = number of metabolites in the pathway, k = number of significant metabolites in the pathway, n = total number of significant metabolites and N = total number of metabolites.