Figure 5

NR3C1 localizes to oocytes and somatic cells in the rhesus macaque periovulatory follicle. (a) NR3C1 IHC was performed on rhesus macaque periovulatory follicles pre- (0 h) and 12 h, 24 h and 36 h post-hCG administration. Positive immunolabeling (brown) was detected in the oocyte, CCs, mGCs, as well as the theca cells lining the follicle wall. There was an apparent reduction in oocyte-specific NR3C1 staining at 12 h post-hCG and the highest intensity of NR3C1 staining was detected in all cell types at 36 h post-hCG administration. The images shown are representative of N = 3 ovaries obtained from individual animals undergoing a COv protocol at each of the times indicated. O = oocyte, C = cumulus cells, A = antrum, T = theca cells, M = mural granulosa cells, and NC = negative control. (b) NR3C1 IF in rhesus macaque oocytes obtained from follicles before (0 h) and 36 h after hCG administration demonstrated NR3C1 localization (green) in both the cytoplasm and nucleus of non-luteinized GV oocytes, but only in the nucleus of luteinizing GV oocytes and CCs that remained attached. Note that NR3C1 also localized to the metaphase plate of mature MII oocytes and that GV oocytes from non-luteinized follicles that reinitiated meiosis by IVM exhibited only cytoplasmic NR3C1 expression. DAPI (blue) was used as a marker for DNA.