Figure 2
Survival (A) and differential gene expression profiles (B,C) of heat-stressed D. melanogaster flies maintained according to the feeding protocols C and AO. (A) Data are least square means ± SEM of flies survived the challenge of 39 °C heat exposure for 75 min after a subsequent recovery period of 24 h (*effect of AO, P < 0.0001). Results are from 9 independent experiments performed in two-to forth-fold determination with 18–22 flies per vial (N = 500 per condition). (B,C) Female, age-matched 10-day old D. melanogaster flies were placed in groups of 18–22 per vial containing control or AO medium and subsequently exposed to sub-lethal heat stress at 39 °C for 60 min. Data are log2 fold change of differentially expressed genes after (B) 60 min of heat stress (t60) or (C) subsequent 180 min of recovery (t240) from the challenge (n = 5/time point). Labeled, gray colored genes were affected by AO (adjusted P < 0.05).
