Table 3 Relative gene expression of cytokines before and after ex-vivo lipopolysaccharide (LPS) stimulation of whole blood taken from lactating heat-stressed cows fed different doses of an Aspergillus oryzae (AO) postbiotic on day 26 of the study. Data are least squares means (LSM) ± SEM. CTL control treatment, IL-1β interleukin 1β, IL-6 interleukin 6, TNF-α tumor necrosis factor-α, Ratio ratio of after-to-before LPS stimulation. a,bSuperscripts denote AO dose differences among LSM (0.05 < P ≤ 0.10). CTL vs. AO denotes contrast between CTL and AO postbiotic. Data were log transformed for statistical analysis and non-transformed LSM are reported.
Item | AO dose, g/day | SEM | P-value | Polynomial contrasts (P-value) | |||
|---|---|---|---|---|---|---|---|
0 | 3 | 6 | CTL vs. AO | Linear | Quadratic | ||
Before LPS | |||||||
IL-1β | 0.156 | 0.198 | 0.315 | 0.103 | 0.851 | 0.273 | 0.775 |
IL-6 | 0.134 | 0.136 | 0.267 | 0.089 | 0.859 | 0.191 | 0.480 |
TNF-α | 0.535 | 0.790 | 0.773 | 0.165 | 0.235 | 0.309 | 0.513 |
After LPS | |||||||
IL-1β | 0.536 | 0.717 | 0.414 | 0.307 | 0.938 | 0.568 | 0.693 |
IL-6 | 2.66 | 2.08 | 2.60 | 0.684 | 0.511 | 0.963 | 0.524 |
TNF-α | 1.87 | 2.76 | 2.47 | 0.749 | 0.584 | 0.565 | 0.530 |
Ratio | |||||||
IL-1β | 2.77 | 3.15 | 2.50 | 0.685 | 0.997 | 0.789 | 0.546 |
IL-6 | 8.17a | 5.92ab | 2.81b | 1.64 | 0.070 | 0.022 | 0.833 |
TNF-α | 0.519 | 0.509 | 0.503 | 0.153 | 0.838 | 0.938 | 0.990 |