Table 3 Relative gene expression of cytokines before and after ex-vivo lipopolysaccharide (LPS) stimulation of whole blood taken from lactating heat-stressed cows fed different doses of an Aspergillus oryzae (AO) postbiotic on day 26 of the study. Data are least squares means (LSM) ± SEM. CTL control treatment, IL-1β interleukin 1β, IL-6 interleukin 6, TNF-α tumor necrosis factor-α, Ratio ratio of after-to-before LPS stimulation. a,bSuperscripts denote AO dose differences among LSM (0.05 < P ≤ 0.10). CTL vs. AO denotes contrast between CTL and AO postbiotic. Data were log transformed for statistical analysis and non-transformed LSM are reported.

From: A postbiotic from Aspergillus oryzae attenuates the impact of heat stress in ectothermic and endothermic organisms

Item

AO dose, g/day

SEM

P-value

Polynomial contrasts (P-value)

0

3

6

CTL vs. AO

Linear

Quadratic

Before LPS

IL-1β

0.156

0.198

0.315

0.103

0.851

0.273

0.775

IL-6

0.134

0.136

0.267

0.089

0.859

0.191

0.480

TNF-α

0.535

0.790

0.773

0.165

0.235

0.309

0.513

After LPS

IL-1β

0.536

0.717

0.414

0.307

0.938

0.568

0.693

IL-6

2.66

2.08

2.60

0.684

0.511

0.963

0.524

TNF-α

1.87

2.76

2.47

0.749

0.584

0.565

0.530

Ratio

IL-1β

2.77

3.15

2.50

0.685

0.997

0.789

0.546

IL-6

8.17a

5.92ab

2.81b

1.64

0.070

0.022

0.833

TNF-α

0.519

0.509

0.503

0.153

0.838

0.938

0.990