Figure 2
CLSM images of S. aureus biofilms exposed to the three substances tested. Representative 3D images of biofilms from MS3 isolate grown statically for 24 h and treated or untreated with subtilisin A (0.01 U/mL), CaG (Ca2+ 1.25 mmol/L), OTC (10 μg/mL) alone or in combination for 24 h. Biofilms were stained with the Syto9/PI, SYPRO Ruby biofilm matrix stain and wheat germ agglutinin (WGA). Scale bars, 30 μm.
