Figure 6 | Scientific Reports

Figure 6

From: Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

Figure 6

Quercetin reduces CCL-2 and IL-1β pro-inflammatory mediators production in ONNV infected HSF cells. HSF cells were infected by ONNV (MOI 1) and/or treated with phenolics (10 µM) for 24 h. Then, RNA was collected and CCL-2 and IL-1β gene expression levels were determined by qRT-PCR, (A) Pro-inflammatory mediators RNA levels. Then, culture media were collected CCL-2 and IL-1β levels evaluated by ELISA, (B) Pro-inflammatory mediators levels. Reported values are means ± SEM of three independent experiments. p value was calculated using the Bonferroni multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001 when compared to non-infected and non-treated cells (CTRL) and #p < 0.05, ##p < 0.01, ###p < 0.001 when compared to infected cells (+ ONNV).

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