Figure 4

Phylogenetic analysis of Leptospira spp. from bonobo fecal samples. The evolutionary history, based on LipL 41 partial gene, was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model. Initial treefor the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. Sequences are identified as follows: accession number/species/strain/host/country. Obtained sequences in the present study showed almost similarity in each other and with Leptospira interrogans serovar Grippotyphosa RTCC2825 (KJ398170). The analysis involved 31 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 436 positions in the final dataset. Evolutionary analyses were conducted in MEGA7⁶¹.