Figure 4
Macrophage cytokine profiles indicate a mixed neuroinflammatory phenotype. Supernatants from treated BMDMs were collected to measure pro- and anti-inflammatory cytokine production in response to phenotype, myelin stimulation, and cPLA2 inhibition with PACOCF3. (A, B) Myelin significanty increases the production of TNF-alpha in M1 cells, but has no effect on CTL and M2 cells. cPLA2 inhibition significantly reduced myelin potentiation of M1 TNF-alpha production. (C, D) Myelin increases CX3CL1 in CTL, M1, and M2 cells. cPLA2 inhibition significantly reduced this effect in M1 cells, but not under M1 and Myelin co-stimulation. (E, F) Myelin significantly decreased IL-10 production in M1 cells, but this effect was not reversed with cPLA2 inhibition. cPLA2 inhibition alone increased IL-10 production in M1 cells. (G, H) Myelin significantly reduced pro-inflammatory IL-1beta production in M1 cells, whereas CTL and M2 cells were unaffected. cPLA2 inhibition did not alter this effect. (I, J) Pro-inflammatory IL-6 was not significantly altered by myelin or cPLA2 inhibition. Refer to Supplementary Fig. 5 for CTL and M2 cPLA2 inhibition data. Representative of three biological replications. *p < 0.05 **p < 0.01, ***p < 0.001 mean ± SEM.
