Figure 2 | Scientific Reports

Figure 2

From: Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery

Figure 2

Change in target transcript abundance at different time points after feeding Leptinotarsa decemlineata larvae with potato leaves coated with dsRNA or a drop containing dsRNA. (A) Transcript abundance (relative to levels in larvae fed untreated potato leaves) in larvae from susceptible (GC) and dsRNA-resistant (CEAS 300) strains when fed potato leaves coated in a solution with 400 μg/ml of dsRNA targeting the V-ATPase subunit A transcript. Asterisks denote significant differences from untreated controls within each population (One Way Analysis of Variance followed by Holm-Sidak’s pairwise multiple comparisons test, P < 0.05). (B) Transcript abundance (relative to time 0 within each strain) when L. decemlineata larvae from susceptible (GC and METT) and dsRNA-resistant (CEAS 300) strains were treated with 25 μg of dsRNA targeting the V-ATPase subunit A transcript. Asterisks denote significant differences from time 0 (control) within each population (Kruskall-Wallis ANOVA on Ranks followed by a Dunnet’s pairwise multiple comparison to the control group, P < 0.05).

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