Figure 3 | Scientific Reports

Figure 3

From: Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery

Figure 3

Cross-resistance to COPI β (coatomer subunit beta) dsRNA in CEAS 300. (A) Changes in target transcript abundance 48 h post-feeding on a drop containing 25 μg of COPI β dsRNA detected by qRT-PCR. Larvae from susceptible (GC and METT) and resistant (CEAS 300) Leptinotarsa decemlineata colonies were treated and their gut dissected after 48 h. Asterisks denote significant difference from time 0 within each population (t-test, P < 0.05). (B) Percentage mortality when larvae were exposed to 600 µg/ml of dsRNA targeting the V-ATPase subunit A (ATP) or COPI β (COPI) transcript compared to control (CONT) treatment during the larval stage. Mortality was assessed during adult emergence.

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