Table 3 Percentage mortality (± standard error, SE) observed in progenies from different crosses when exposed to 800 µg/ml of V-ATPase subunit A dsRNA in 20-day leaf-feeding bioassays. These mortalities were used to determine the genetics of resistance in CEAS 300.
Test | Na | Control mortality (%) ± SE | Corrected treatment mortality (%) ± SE |
|---|---|---|---|
F1 bioassay 1—GC and CEAS 300 | |||
GC (♀) × GC (♂) | 4 | 1.7 ± 1.7 | 56.6 ± 10.0 |
GC (♀) × CEAS 300 (♂) | 4 | 1.7 ± 1.7 | 31.7 ± 5.7 |
CEAS 300 (♀) × GC (♂) | 4 | 1.7 ± 1.7 | 40.0 ± 7.7 |
CEAS 300 (♀) × CEAS 300 (♂) | 4 | 1.7 ± 1.7 | 1.7 ± 1.7 |
F1 bioassay 2—METT and CEAS 300 | |||
METT (♀) × METT (♂) | 8 | 11.7 ± 4.8 | 100.0 ± 0.0 |
METT (♀) × CEAS 300 (♂) | 4 | 23.4 ± 1.9 | 95.6 ± 3.3 |
CEAS 300 (♀) × METT (♂) | 8 | 8.3 ± 2.1 | 94.0 ± 2.1 |
CEAS 300 (♀) × CEAS 300 (♂) | 8 | 4.2 ± 1.7 | 7.7 ± 1.7 |
Backcross bioassay—METT, CEAS 300 and F1 | |||
METT (♀) × METT (♂) | 8 | 6.6 ± 4.2 | 98.0 ± 1.7 |
METT (♀) × F1b (♂) | 8 | 10.8 ± 4.5 | 73.7 ± 7.2 |
F1 (♀) × METT (♂) | 4 | 6.7 ± 2.7 | 96.4 ± 1.9 |
CEAS 300 (♀) × F1 (♂) | 4 | 0.0 ± 0.0 | 36.6 ± 4.3 |
CEAS 300 (♀) × CEAS 300 (♂) | 4 | 5.0 ± 3.2 | 13.3 ± 9.4 |
